General Information

  • ID:  hor004711
  • Uniprot ID:  Q84MD2
  • Protein name:  Protein CASPARIAN STRIP INTEGRITY FACTOR 1
  • Gene name:  CIF1
  • Organism:  Arabidopsis thaliana (Mouse-ear cress)
  • Family:  NA
  • Source:  Plant
  • Expression:  Induced by excess iron and further synergistically regulated by lowering the medium pH. |Accumulates in the stele, especially at the phloem pole, of the mature region of the primary roots. |Expressed exclusively in the root stele.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Arabidopsis (genus), Camelineae (tribe), Brassicaceae (family), Brassicales (order), malvids, rosids, Pentapetalae, Gunneridae, eudicotyledons, Mesangiospermae, Magnoliopsida (class), Spermatophyta, Euphyllophyta, Tracheophyta, Embryophyta, Streptophytina (subphylum), Streptophyta (phylum), Viridiplantae (kingdom), Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0005179 hormone activity; GO:0005515 protein binding
  • GO BP:  GO:0007165 signal transduction; GO:0071555 cell wall organization; GO:0160073 Casparian strip assembly; GO:2000067 regulation of root morphogenesis
  • GO CC:  GO:0048226 Casparian strip

Sequence Information

  • Sequence:  LSVSFAGRPSIFVHKKINLREEMVERSMHEHERLLRMNTKDYGNNSPSPRLERPPFKLIPN
  • Length:  61(23-83)
  • Propeptide:  MGMSPLTVKKLGFIFMIVSASALSVSFAGRPSIFVHKKINLREEMVERSMHEHERLLRMNTKDYGNNSPSPRLERPPFKLIPN
  • Signal peptide:  MGMSPLTVKKLGFIFMIVSASA
  • Modification:  T42 Sulfotyrosine;T47 Hydroxyproline;T49 Hydroxyproline
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Peptide hormone required for contiguous Casparian strip diffusion barrier formation in roots via the regulation of CASPs protein expression and distribution in a GSO1-GSO2 signaling pathway. The Casparian strip is required for ion homeostasis (e.g. iron a
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-Q84MD2-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor004711_AF2.pdbhor004711_ESM.pdb

Physical Information

Mass: 827165 Formula: C317H511N97O89S3
Absent amino acids: CQW Common amino acids: RELPS
pI: 10.81 Basic residues: 14
Polar residues: 15 Hydrophobic residues: 16
Hydrophobicity: -83.77 Boman Index: -17043
Half-Life: 5.5 hour Half-Life Yeast: 3 min
Half-Life E.Coli: 2 min Aliphatic Index 73.44
Instability Index: 6280.66 Extinction Coefficient cystines: 1490
Absorbance 280nm: 24.83

Literature

  • PubMed ID:  28104889
  • Title:  A peptide hormone required for Casparian strip diffusion barrier formation in Arabidopsis roots.